Liquid Biopsy New Technology, Like finding a needle in a haystack
U of T researchers develop liquid biopsy technology for prostate cancer
Researchers at the University of Toronto have developed a new technology for a liquid biopsy to identify which patients might not respond to standard therapy for prostate cancer before it is delivered.
What is liquid biopsy?
A liquid biopsy is a simple and non-invasive alternative to surgical biopsies which enables doctors to discover a range of information about a tumour through a simple blood sample. Traces of the cancer’s DNA in the blood can give clues about which treatments are most likely to work for that patient.
Prostate cancer is the most common cancer among men (after skin cancer), but it can often be treated successfully. If you have prostate cancer or are close to someone who does, knowing what to expect can help you cope. Here you can find out all about prostate cancer, including risk factors, symptoms, how it is found, and how it is treated.
Prostate cancer is the second most common cancer in men and the fifth leading cause of death from cancer in men worldwide, according to 2012 numbers. While several viable treatment options for prostate cancer exist, many men affected with prostate cancer will not respond to first-line treatments.
“Screening for drug resistance is key to improving treatment approaches for many cancers,” said Shana Kelley, a professor at U of T’s Leslie Dan Faculty of Pharmacy. “It’s important for patients not to be on a therapy that won’t help them and it’s also important for health-care systems to avoid, whenever possible, delivering ineffective treatments.”
The ability to screen patients using a blood sample as opposed to more invasive techniques required for conventional biopsies is also a step forward. Liquid Biopsy is a simple and non-invasive alternative to surgical biopsies which enables doctors to discover a range of information about a tumor through a simple blood sample.
Kelley, lead investigator on the study published today in Nature Chemistry, explained how her team has advanced a completely new approach, using magnetic nanoparticles with DNA capture probes on their surface that can target circulating tumour cells (CTCs) in blood samples to see if the cells contains biomarkers associated with drug resistance.
“We can then trap the individual magnetized cells in a microfluidic device built in the lab, isolating them from all the other cells in the sample and allowing us to perform highly sensitive analysis,” Kelley said. The cells with the highest magnetic content will also have high mRNA expression for the biomarker associated with drug resistance.
“This means that patients with high mRNA expression should be considered for other therapies because they won’t respond to the first-line treatment.”
Liquid Biopsy proves to be a promising tool for the management of Cancer
Targeting CTCs, the cells responsible for spreading cancer, is important because they carry information from the primary tumour that can inform treatment. They are, however, outnumbered by a billion-to-one by normal cells in a patient’s blood and are therefore extremely challenging to capture.
In 2016, Kelley and her team published a study in Nature Nanotechnology that first introduced the microfluidic device and how it could be used to trap and analyze CTCs. The current study builds on this previous work by further targeting a specific biomarker within the CTCs.
The blood samples analyzed were collected from a small cohort of patients undergoing treatment for metastatic prostate cancer. In 10 of the patients tested, CTCs were visualized but only four of the patients exhibited the biomarker associated with drug resistance. This finding demonstrates that the new method can provide both a CTC count and an analysis of the clinically relevant biomarker.
“We are very excited because this is like finding a needle in a haystack. It paves the way for a straightforward and personalized screening tool that allows clinicians to see if a patient will respond to therapy or not. The liquid biopsy method is also rapid, accurate and inexpensive, which gives it real potential for clinical uptake,” said Kelley.
As for next steps, the finding must be replicated in a larger study, Kelley said. Her team is also focused on “scaling up” and expanding the application of this technology to other forms of cancer and other diseases.
“Liquid biopsy is one of the most promising tools emerging for the management of cancer,” said Kelley. “We are excited about the potential of our technology to streamline this type of testing.”
The research was supported by the Canadian Institutes of Health Research, the Natural Sciences and Engineering Research Council of Canada, Ontario’s Ministry of Research, Innovation and Science, and the National Cancer Institute of the National Institutes of Health.
Article Source: University of Toronto
April 02, 2018 – Article by Kate Richards
About University of Toronto
Founded in 1827, the University of Toronto has evolved into Canada’s leading institution of learning, discovery and knowledge creation. We are proud to be one of the world’s top research-intensive universities, driven to invent and innovate.
Our students have the opportunity to learn from and work with preeminent thought leaders through our multidisciplinary network of teaching and research faculty, alumni and partners.
The ideas, innovations and actions of more than 560,000 graduates continue to have a positive impact on the world.